Bolted rabbet joint structure is commonly used in aero-engine, and bolt pre-tightening force is usually dispersed after assembled under the current process technology, which can aggravate the unsteadiness of mechanical properties. This paper analyzes the Von Mises equivalent stress of the joint interface and stiffness of the bolted rabbet joint structure with FEA software ANSYS Workbench. Five levels of bolt pre-tightening force dispersion were set up by engineering experience, then a group of pre-tightening forces were randomly generated for each of the dispersion level to simulate the pre-tightening force in engineering condition. The stress distribution of joint interface, the axial stiffness, the radial stiffness and the torsional stiffness were calculated, and related mathematical relation curves were plotted. Finally, the influence rules of bolt pre-tightening force dispersion on the bolted rabbet joint structure were summarized which can provide a theoretical reference for the control of bolt pre-tightening force dispersion.