According to the measurement technology requirements of aircraft assembly for large-volume, high precision, strong compatibility, good expansibility and fast networking, the research on establishment technology of large-volume multi-system measurement field for aircraft assembly is carried out, the principle of measuring reference optimization design is proposed and the multi station redundant measurement length constraint algorithm is adopted based on laser tracker. A 3D precision reference measurement field compatible with aircraft assembly equipment is constructed, and the calibration accuracy of the reference field is verified by experimental comparison. Eventually, through a typical application case, this paper expounds the construction technology of large-volume multi-system measurement filed in the process of aircraft assembly, which promotes the improvement of aircraft assembly measurement technology.