In order to continuously improve the operational performance of the aircraft final assembly production line and promote the reform of the business model, this paper proposed a new management and control mode of aircraft final assembly production line based on digital twin. Firstly, the evolution process of aircraft final assembly production line from physical space to information space is analyzed，as well as the operation control requirements of production line. Secondly, the digital twin composition of aircraft final assembly production line is expounded, at the same time, the digital twin model of assembly production line is constructed based on five-dimension digital twin model. Finally, the virtual model is established based on 3DMAX technology, and a digital twin system for final assembly is built by system development technology. The mapping between physical entity and virtual space is realized by collecting the process data of production line, and the digital twin technology in aircraft final assembly production line is preliminarily applied. The results show that the control mode of aircraft final assembly production line based on digital twin plays an important role in improving production efficiency and quality.