Large civil aircraft has a feature of dispersed production base and high assembly quality requirements, the problem that uncoordinated assembly occurs in the process of join-assembly will result in the delivery extension and higher costs. The reasonable crafts technology and repair ability of the parts with problems are the key points of ensuring the final assembly quality. We combine the virtual assembly technology and digital measurement technology, collect the actual shape and size data of key features of different fuselage segment assembly, achieve the purpose of virtual assembly by fitting the different fuselage segment measurement data into one same fuselage coordinate system, calculate the gap, adjacent difference,angle and other GD & T parameter with the result of virtual assembly, evaluate the assembly quality and provide solution and repair amount for the existing issues of the evaluation results. Firstly, we propose the technology of the fuselage segments virtual assembly based on measurement data, secondly research the key points of the technology and use it in one aircraft fuselage join-assembly process. The application of this technology reduces the failure rates and improves the final join-assembly speed, which gets one good effect.